Gene discovery in Trypanosoma carassii.

A web appendix following the publicacion of:

Gene discovery in the freshwater fish parasite Trypanosoma carassii: identification of trans-sialidase like and mucin like genes.

Infection and Immunity 70 (2002): 7140-7144.

Fernán Agüero(1,#), Vanina Campo(1,#), Laura Cremona(1,§), Adriana Jäger(1), Javier M. Di Noia(1,*), Peter Overath(2), Daniel O. Sánchez(1,&) and Alberto Carlos Frasch(1,&).

(1) Instituto de Investigaciones Biotecnológicas,
Universidad Nacional de General San Martín,
1650, San Martín, Argentina.

(2) Max-Planck-Institut fur Biologie, Abteilung Membranbiochemie
Correnstrasse 38, D-72076, Tübingen, Germany.

(#) Fernán Agüero and Vanina Campo contributed equally to this work.
(&) Corresponding authors.
(§) Present address: Cellular Biochemistry and Biophysics, Memorial Sloan-Kettering Cancer Center, 1275 NY Ave., Box 251, New York, NY, 10021, USA.
(*) Present address: MRC Laboratory of Molecular Biology. PNAC Unit, Hills Road Cambridge CB2 2QH, Cambridge, United Kingdom.

Web appendix

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Supplement to Figure 1.

Alignment of TcaTS and TcTS

T. cruzi transialidase (TcTS) and the T. carassii TS-like (TcaTS) were aligned together using clustalw. The portion of the alignment shown includes the mature catalytic domain only. Identity between residues is indicated with stars (*), conservative changes are indicated with double dots (:), less conservative changes are indicated with a single dot (.). Boxes highlight the residues involved in the catalytic centre of the sialidases of known crystal structure. I, II, and III are conserved aspartic boxes. NA is a signature motif of the neuramindase (NA) superfamily.

Gene discovery: new genes

Results of BLASTX analysis against protein databases. We have divided matches in two categories:

The information is presented in a non-redundant form (matches to the same or similar database entries have been merged together). A full table (redundant) can be downloaded in tab-delimited (.txt) or spreadsheet (MS Excel, .xls) format.

Sequence summaries

All sequences have been subjected to similarity searches against different databases, and to testcode evaluation of their coding potential. A summary of the results for each sequence is available.

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If you are looking for a specific clone (e.g. clone 02j2) use the form below:

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Fernán Agüero, 26.Aug.2002 - 11:54:07 ART